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The fluorophore is an excellent candidate for displaying Acridine orange is excited by blue-green laser light (488 nanometers) or by ultraviolet, violet, and blue interference filters coupled to an arc-discharge lamp (mercury or xenon). Acridine Orange - A nitrogen heterocyclic chromophore containing the acridine nucleus, which binds to DNA and RNA by intercalation between successive base pairs to produce a strong, but very broad emission band in the green to red wavelength region.Specific wavelengths are selected by altering the frequency of the sound waves delivered to the crystal, thus changing the period of the diffraction grating. Passes through the filter, the crystal acts as a diffraction grating to deflect the incident beam.
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As either polarized or non-polarized light The filter consists of a specialized crystal, such as tellurium oxide or quartz, sandwiched between an acoustic transducer and absorber to induce standing sound waves with alternating domains of high and low refractive index. Acousto-Optic Tunable Filter (AOTF) - A filtering device that utilizes sound waves to modulate the wavelength or intensity of light emitted by a laser or non-coherent illumination source (primarily arc-discharge lamps).Absorption bands typically cover a wide range (many tens or hundreds) of wavelengths and are commonly plotted as intensity, transmission, or optical density versus Units of absorbance are equivalent to the logarithm of the reciprocal transmittance (the ratio of the transmitted light intensity to incident light intensity). Absorbance (Optical Density) - The quantity of light absorbed by a chemical or biological substance as measured in a spectrophotometer or similar device.This resource is provided as a guide and reference tool for visitors who are exploring the large spectrum of specialized topics in fluorescence and laser scanning confocal microscopy. The complex nomenclature of fluorescence microscopy is often confusing to both beginning students and seasoned research microscopists alike.